01/04/2013 : Curriculum vitae du Professeur Mustapha OUMOUNA
 CURRICULUM VITAE

Mustapha OUMOUNA, M.Sc. Ph.D.

 

Address:

Université Saad Dahlab-Blida

Faculté des Sciences Agro-Vétérinaires

Département des Sciences Véterinaries

B.P: 270 Route des Soumaa-Blida

Phone: +213 (0)25 43 36 37

Email: oumouna@gmail.com

 

Education:

1999 Doctor of Philosophy in Biological Sciences and Fish Parasitology, University of Munich, Germany. Funded by the German Academy for Exchange Services (DAAD).

1991 Master of Science in Biology, University of Technology and Sciences, Algiers (Algeria).

1987 Bachelor of Science in Biology, University of Technology and Sciences, Algiers (Algeria).

Expérience Professionnelle:
Février 1988-Juillet 1989: Assistant en Génétique, Ecole des Sciences Médicales (Université d’Alger). 
Février 1988- Juillet 1989: Assistant en Histologie-Cytologie (Université de Blida), Algérie.Décembre 1991- Juillet 1992: Chargé de cours en Maladie des Poissons (Université de Blida), Algérie
Septembre 1989- Juin 1992: Instructeur en Microscopie électronique, Service de  Microscopie Electronique au Service d’Anatomie Patholgique “Centre de Pierre et Marie Curie” CHU Mustapha Bacha, Alger, Algérie.
Septembre 1991- Juin 1994: Maitre Assistant en Biologie Cellulaire  (Université de Blida), Algerie.
Janvier 1997- Juin 1997:Assistant en Parasitology, Faculty of Veterinary Medicine (Ludwig-Maximillians-University), Munich, Germany.
Octobre 1999-  Juin 2002: Postdoctoral Research Associate in Fish Molecular Immunology, Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, USA.
Aout 2002-  Juin 2004: Postdoctoral Research Associate in Virology and Vaccine Formulations at Vaccine and Infectious Disease Organization, VIDO (University of Saskatchewan) Canada.
Juin 2004-  Aout 2005: Senior Postdoctoral Researcher at the Department of Pharmacology, School of Medicine, Louisiana State University, USA.
Janvier 2006- Septembre.2007: Maitre Assistant en Immunologie et Microbiologie:Departement des Sciences Vétérinaires et LMD. Saad Dahled University Blida, Algeria.

Octobre 2007-Present :Maitre de Conference en  Immunologie et Microbiologie: Département des Sciences Vétérinaires et LMD. Saad Dahled University Blida, Algeria.

Postes occupés et nominations à l’Université Saad Dahleb Blida:                                                                 

Mars 2009- Présent : Responsable du Domaine LMD Sciences de la Nature et de la Vie, Faculté des Sciences Agro-Vétérinaires.

Avril 2010- Présent : Membre du comité Scientifique du Département des Sciences Vétérinaires.

Avril 2010- Présent : Membre du Conseil de Faculté, Faculté des Sciences Agro-Vétérinaires.

Nominations au CNRDPA :

2008- 2011 :Responsable de la Division Aquaculture.

2008- Présent :Chef d’équipe Maitrise des Techniques de Pisciculture Continentale et valorisation des Ressources Hydriques.

2009- Présent :Membre du Conseil Scientifique du CNRDPA.

2009- Présent : Membre du Conseil d’Administration du CNRDPA.
Nomination Ministérielle: 2011- Présent : Membre du Conseil National Scientifique et Technique (parmi les 14 personnalité scientifique Algérienne).
Guest Scientist:
1994  Guest scientist for two months at Vaccine and Infectious Diseases Organization, Canada(VIDO) (sponsored by VIDO).
2007   Guest scientist for two months at the Department of Hygiene and Microbiology at the University of Heidelberg, Germany (sponsored by the German Academy of Exchange Services DAAD).
2008-2011 Guest scientist for two months at the Department of Hygiene and Microbiology at      the University of Heidelberg, Germany (sponsored by the Department of Hygiene and Microbiology, Gemany).
Teaching activities:
Genetics: Taught first year student in Human Medicine program.
Histology and Cytology: Taught basic histology and cell biology, mainly cell ultra-structure, as well as the laboratory portion and demonstration of all technique for preparation of the samples for light- and electron microscope.
Cell Biology (undergraduate level course): Taught the ultra-structure and the physiology of the cell. Detail description of all the component of the cell and their function.
Fish Disease: Mainly fish parasitology and the consequences of some diseases in aquaculture.
Parasitology: Contribution in the preparation and assisting in teaching of Parasitology course for students in Veterinary Medicine (Germany).
Immunology and Microbiology: Taught second year student in Veterinary Medicine and LMD.
Methodolgy: Scientific articles in English for Master students.

Technical Expertise: Expertise acquired in a variety of scientific fields (Virology, Parasitology, Immunology, Molecular Biology, Immunohistoschemistry, Immunofluorescence, and Histochemistry, Cell Ultrastructure,  Scanning and Transmission Electron Microscopy, Flow Cytometry). Some of these include a number of assays for apoptosis (annexin V staining, NIM (Nuclear Isolation Media), etc…); immunology (teleost innate immunity, mammalian immunity, DNA vaccine using bacterial genomic DNA and synthetic oligonucleotides); vaccine development (preparation of formalin inactivated vaccine for Human and Bovine Respiratory Syncytial Virus, HRSV and BRSV respectively), general molecular biology (Western blots, DNA cloning, PCR, transfection, immunoprecipitation, electrophoresis, in vitro transcription/translation, etc…); cell biology (cytotoxicity and cell viability assays, immunofluorescence, cell culture, preparation of primary cell cultures), Flow cytometry (Tow color analysis, annexin V, PI, NIM and cytotoxicity); biochemistry (Preparation and purification of monoclonal antibody and protein purification); and in addition to many others.

Animal model experience: Fish, Mouse, Cattle.

Special Recognition:
1994-1999     Awarded the German Scholarship of the Exchange Services (DAAD).

Languages:  Arabic, English, French, German
Public Service:1987-1994:    President of Scientific Association “Knowledge” (Algeria).

Trainings:
1990 Training on electron microscopy, Faculty of Veterinary Medicine, Munich (Germany).
1993 Fish Parsitology training, Faculty of Veterinary Medicine, Munich (Germany).
1994 Fish Parasitology and electron Microscopy training, Faculty of Veterinary Medicine, Munich (Germany).
1996 Invertebrates systematic of species occurring in Germany withspecial reference to the immature stages. Organized by the German Society of Limnology.

Publications:

1.        Oumouna, M., El-Matbouli, M. and Hoffmann, R. (1996) Occurrence of actinosporean in a trout farm enzootic for Proliferative Kidney Disease (PKD). Light and electronmicroscopy (German). German Veterinary Medical Society 125-133.

2.        A. Bretzinger, T. Fischer-Scherl, M. Oumouna, R. Hoffmann and U. Truyen (1999) Mass mortalities in Koi Carp, Cyprinus carpio, associated with skin and gill disease. Bull. Eur. Ass. Fish Pathol. 19(5), 182-185.

3.        OumounaM., Mansour El-Matbouli, Rudolf W. Hoffmann and Georges Bouix (2000) Electron microscopic study of a new microsporeanMicrosporidium epithelialis sp. n. infecting Tubifex sp. (Oligochaete). Folia Parasitologica 47: 257-265.

4.        Donald Evans, Mustapha Oumouna & Liliana Jaso-Friedmann (2000) Nonradiometric detection of cytotoxicity of teleost nonspecific cytotoxic cells. Methods in Cell Science. 22: 233-237.

5.        M. Oumouna, L. Jaso-Friedmann and D.L. Evans (2001) A Flow Cytometry Assay for Determination of Teleost Cytotoxic Cell Lysis of Target Cells. Cytometry 45: 259-266.

6.        M. Oumouna, M. El-Matbouli, R.W. Hoffmann and S.L. Hallett (2002) Early developmental stages of two actinosporeans, Raabeia and Aurantiactinomyxon (Myxozoa), as detected by light and electron microscopy.Journal of Invertebrate Pathology 79: 17-26.

7.        M. Oumouna, L. Jaso-Friedmann and D.L. Evans (2002) Activation of Cytotoxic Cells (NCC) with Synthetic Oligonucleotides and Bacterial Genomic DNA: Binding, Specificity and Identification of Unique Immunostimulatory Motifs. Developmental and Comparative Immunology 26: 257-269.

8.        M. Oumouna, S. L. Hallett, R. W. Hoffmann, and M. El-Matbouli (2003) Seasonal occurrence of actinosporeans (Myxozoa) and oligochaetes (Annelida) from a Trout hatchery in Bavaria, Germany. Parasitology Research 89: 170-189.

9.        M. Oumouna, J. Mapeltoft, B. Karvonen, L. Babiuk and S. van Den Hurk (2005). Effects of CpG oligonucleotides (ODN) adjuvants on immune responses and protection induced by bovine respiratory syncytial virus (BRSV) formalin inactivated (FI) or commercial killed vaccines (K-BRSV). Journal of Virology 79: 2024-2032.

 

10.     John W. Mapletoft, Mustapha OumounaHugh G. Townsend, Lorne A. Babiuk, Sylvia van Drunen Little-van den Hurk. (2005). Formulation with CpG oligodeoxynucleotides increases cellular immunity induced by vaccination with formalin-inactivated bovine respiratory syncytial virus. Virology 353:316-23.

11.     Boulares H, Zoltoski A, Kandan S, Akbulut T, Yakovlev A, Oumouna M (2006) Correlation between decreased sensitivity of the Daudi lymphoma Cell to VP-16-induced apoptosis and deficiency in DNAS1L3 expression. Bichem Biophys Res Commun 341: 653-662.

12.     Oumouna Mustapha, Rahul Datta, Karine Oumouna-Benachour, Yasuhiro Suzuki, Kametra Matthews, Kenneth Fallon and A. Hamid Boulares (2006). Poly(ADP-ribose) Polymerase-1 Inhibition Prevents Eosinophil Recruitment by Modulating Th2 Cytokines in a Murine Model of Allergic Airway Inflammation: A Potential Specific Effect on IL-5. Journal of Immunology 177: 6489-6496.

13.     Karine Oumouna-Benachour, Mustapha Oumouna, Mourad Zerfaoui, Chetan Hans, Kenneth Fallon, A. Hamid Boulares (2007). Intrinsic resistance to apoptosis of colon epithelial cells is a potential determining factor in the susceptibility of the A/J mouse strain to dimethylhydrazine-induced colon tumorigenesis. Molecular carcenogenesis 115: 2442-2450.

14.      John W. Mapletoft, Mustapha Oumouna, Jennifer Kovacs-Nolan, Laura Latimer, George Mutwiri, Lorne A. Babiuk, and Sylvia van Drunen Littel-van den Hurk (2008). Intranasal immunization of mice with a formalin-inactivated. bovine respiratory syncytial virus vaccine co-formulated with CpG oligodeoxynucleotides and polyphosphazenes results in enhanced protection. Journal of General Virology 89: 250-260.

15.     Naura AS, Hans CP, Zerfaoui M, You D, Cormier SA, Oumouna M, Boulares AH (2008). Post-allergen Challenge Inhibition of Poly(ADP-ribose) Polymerase Harbors Therapeutic Potential for treatment of Allergic Airway Inflammation. Clinical and Experimental Allergy 38: 839-846.

16.      Naura AS, Datta R, Hans CP, Zerfaoui M, Rezk BM, Errami Y, Oumouna M, Matrougui K, Boulares H (2009). Reciprocal regulation of iNOS and PARP-1 during allergen-induced eosinophilia. European Respiratory Journal 33: 252-262.

17.      Van den Broeke A, Oumouna M, Beskorwayne T, Szynal M, Cleuter Y, Babiuk S, Bagnis C, Martiat P, Burny A and Griebel P (2010). DNA Vaccination Induces Tax-Specific Cytotoxic Cells, Alters Bovine Leukemia VirusReplication but Does not Prevent Oncogenesis. Leukemia Research 34: 1663-1669.

18.     Rahul Datta, Amarjit S. Naura, Mourad Zerfaoui, Mustapha Oumouna, Hogyoung Kim, Jihang Ju, Youssef Errami, and A. Hamid Boulares (2011). PARP-1 deficiency blocks IL-5 expression through calpain-dependent degradation of STAT-6 in a murine asthma model. Allergy 7: 853-861.

Oral Presentations;

Oumouna, M. and Benmeradi, N. (1990) Preliminary results about the reproduction cycle of Sepia officinalis L. 1st. Maghrebin Meeting of Marine Science. Algeria, Sidi-Fredj, 3-5 May.

Benmeradi, N. and Oumouna, M. (1990) Influence of diet on the growth of mussel larvae, Perna perna L. 1st. Maghrebin Meeting of Marine Science. Algeria, Sidi-Fredj, 3-5 May.

Oumouna, M. and Benmeradi, N. (1993) Structural and ultrastructural evolution of the accessory nidamentale glands during the reproduction cycle of Sepia officinalis L.Annual Meeting of the French Society of Zoology. France, Bordeaux 5-7 July.

Oumouna, M., El-Matbouli, M. and Hoffmann, R. (1996) Occurrence of actinosporean in a trout farm enzootic for Proliferative Kidney Disease (PKD).Meeting of the German Veterinary Medical Society and the German Section of the European Association of Fish Pathologists (EAFP). Germany, Königswartha, 24-26 September.

Oumouna, M., El-Matbouli, M. and Hoffmann, R. (1999) Actinosporeans in a trout farm for Proliferative Kidney Disease (PKD). V International Symposium of Fish Parasites. Czech republic, Ceske Budejovice 9-13 August.

Oumouna, M., Friedmann-Jaso, L. and Evans, DL. (2002) Binding of Bacterial Genomic DNA and Synthetic Oligonucleotides leads to Activation of Tilapia Nonspecific Cells (NCC). 27th Eastern Fish Health Workshop, USA, South Carolina, 18-22 March.

Oumouna, M., J. Mapeltoft, B. Karvonen, L. Babiuk and S. van Den Hurk (2004). Effects of CpG oligonucleotides (ODN) adjuvants on immune responses and protection induced by bovine respiratory syncytial virus (BRSV) formalin inactivated (FI) or commercial killed vaccines (K-BRSV). 23rd Annual Meeting of American Society for Virology. Canada, Montreal, 10-14 July.

Abstract Presentations:

Oumouna, M., El-Matbouli, M. and Hoffmann, R. (1995) Occurrence of Actinosporea in trout farm with a long history of Proliferative Kidney Disease (PKD).VI International Symposium of Fish ParasitologyGermany, Munich 3-7 October.

M. Oumouna, L. Jaso-Friedmann, H. Kaur and D. L. Evans (2001) Mechanisms of Innate Immunity in Teleost: Activation of Cytotoxicity with Bacterial DNA and Synthetic Oligodeoxynucleotides. Annual Meeting of the American Association of Immunologists (2001). USA, Orlando, Florida, March31-April 4.

H. Kaur, M. Oumouna, L. Jaso-Friedmann and D. L. Evans (2001) Role of Bacterial DNA and Oligonucleotides Motifs in Innate Immune Defense Mechanisms in Fish. Veterinary Research Day. USA, Athens, Georgia, February 12th.

D. L. Evans, M. Oumouna, Harjeet Kaur and L. Jaso-Friedman (2001) Activation of Nonspecific Cytotoxic Cells (NCC) by Novel Immunostimulatory OligodeoxynucleotidesThe 5th Nordic Symposium on Fish Immunolog. Norway, Sundvolden, 18-22 June.

D. L. Evans, M. Oumouna, and L. Jaso-Friedman (2002) In Vivo Activation of Nonspecific Cytotoxic Cells (NCC) with Oligodeoxynucleotides and Bacterial Genomic DNA. Annual Meeting of the American Association of Immunologists.USA, Orleans, Louisiana, April 19- 23.

Hamid Boulares, Yasuhiro Suzuki, Amarjit Naura, Mustapha Oumouna, Rahul Datta, and Chetan Hans (2007). PARP-1 and iNOS relationship in allergen-induced lung inflammation: dependence of iNOS expression on PARP-1 and its dispensability for eosinophil recruitment post-IL-5 production. American Association of immunologists, Miami Beach, Florida, USA, May 18-22.

Mustapha Oumouna, Kametra M. Matthews, Kirk Hutchinson, Rahoul Datta, Kenneth Fallon and Hamid Boulares (2005) Role of PARP-1 in Th2-cytokine secretion and mucus production in a murine model of asthma. Experimental Biology Meeting, USA, San Diego, California, April 2-6.

John W. Mapletoft, Mustapha OumounaHugh G. Townsend, Lorne A. Babiuk,Sylvia van Drunen Little-van den Hurk (2005) Formulation with CpG oligodeoxynucleotides increases cellular immunity induced by vaccination with formalin-inactivated bovine respiratory syncytial virus. Vaccine Congress, Germany, Berlin, September 8-10.

Amarjit Singh Naura, Mustapha Oumouna, Mourad Zerfaoui, Chetan Hans, Yasuhiro Suzuki, and Hamid Boulares (2007) A post-allergen challenge inhibition of PARP prevents airway eosinophilia and reduces Th2 cytokines and mucus production in animal model of asthma:

A clinical potential in the treatment of asthma. American Association of immunologists, Miami Beach, Florida, USA, May 18-22.

Professional Experience

February 1988- July 1991:     

Head of Teaching Assistants in Histology-Cytology at the Veterinary Science Institute (University of Blida), Algeria: I supervised junior teaching assistants and taught basic histology and cell ultra-structure (light- and electron microscopy) to undergraduate students as well as directed the overall operations of the laboratory portion of the course.

February 1988- July 1989:

Teaching Assistant in Genetics, School of Medicine (University of Algiers) Algeria.

December 1991- July 1992:

 Lecturer in Fish Disease for student in veterinary medicine. These are a series of lectures that are taught in the span of one semester. I was fully in charge of this course. The lectures focused mainly on fish physiology and Parasitology and the consequences of some diseases in fish production in aquaculture. 

September 1991- June 1994:

  Lecturer in Cell Biology (University of Blida), Algeria. Undergraduate level course for student at the Veterinary Science Institute. I was fully in charge of this course. I taught the ultra-structure and the physiology of the cell with detail description of all the component of the cell and their functions.

January 1996- June 1997:

Teaching Assistant in Parasitology, Faculty of Veterinary Medicine (Ludwig-Maximillians-University, Munich) Germany.

15th October 1999- 30th June 2002:               

Postdoctoral Research Associate in Molecular Immunology (Department of Infectious Diseases, Faculty of Veterinary Medicine, University of Georgia, USA). My independent studies in Dr Evan’s laboratory focused on characterizing the mechanisms by which Natural Killer cells are activated by synthetic oligonucleotides and bacterial DNA in teleosts (fish) model. This led me to initiate several studies into the development of DNA vaccine against Streptococcus iniae infection. Due to the endogenous processing of antigen as a mechanism to induce an immune response, DNA vaccines have several advantages over conventional vaccines, such as antigen presentation via individual MHC class I and II receptors. Furthermore, DNA vaccines have a high degree of safety and elicit significant immune responses in vertebrates. Further, these vaccines have been already tested, successfully, against several diseases including asthma and diabetes. Recently synthetic DNA was used as a potent immunotherapeutic agent of cancer when injected intratumorally or peritumorally. These studies culminated into several research articles that were published in reputable journals in the field of immunology and parasitology (see CV) as well as others that are in preparation.

26th August 2002- 4th June 2004:

  Postdoctoral Research Associate at the Vaccine and Infectious Disease Organization (VIDO). To further my understanding on the function of CpG motifs (DNA vaccine) in the immune modulation of the mammalian system I moved to the VIDO at the University of Saskatchewan (Canada) to work with Dr. S. Van den Hurk and Dr. L. Babiuk, a pioneer in the field. The goal of my work at VIDO is to use CpG ODNs as adjuvant in formulation with Respiratory Syncytial Virus (RSV) vaccines. I also address the ability of CpG ODN to shift immune responses as well as enhance protection from virus challenge. In addition, my work will contribute to understand the RSV infection at the molecular level.  A good knowledge of the immune response will allow us to interfere with the infection process to achieve improved therapeutic strategies. My initial observations in this independent project have now been published in the Journal of Virology and Virology.

 June 2004 – 15th August 2005:

Senior Postdoctoral Research Associate at the Department of Pharmacology and Experimental Therapeutics at the School of Medicine of Louisiana State University. Allergic asthma and chronic obstructive pulmonary disease are complex respiratory disorders that affect millions of individuals worldwide. To understand the role of the immune system and particularly the delicate balance between Th1 and Th2 response, observed in RSV model as well as in allergic reactions, I joined the Department of Pharmacology at the School of Medicine of Louisiana State University. Indeed, RSV is considered as the most commonly implicated virus linked to viral-induced bronchiolitis with the subsequent development of recurrent wheezing and asthma. In my asthma project I am using knockout mice that lack the expression of some genes, to understand the mechanisms by which the immune system is regulating the response to allergen and particularly the role of some proteins like PARP in the lung inflammation. My initial results have been presented in the Experimental Biology Meeting in San Diego California (April 2005) and published in the international journals in the field of Immunology and Allergies.

 





Conception : VITAMINEDZ